pOTF3-AK (clone3)
(Plasmid #21165)

• Depositing Lab: James Thomson
• Publication: Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Backbone

• Vector backbone: pBluescript SK (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Cre/Lox ; homologous recombination
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: POUF5F1 (genomic DNA)
• Alt name: Oct4
• Species: H. sapiens (human)
• Insert Size (bp): 13500
• Mutation: NeoR, EGFP, and SV40 polyA are between the two genomic sequences (one section is 1604 bp and one is 9022).
• GenBank ID: AC006047
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pLac

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The gene-targeting vector was constructed by insertion of an IRES-EGFP, and IRES-Neo, and an SV40 polyadenylation sequenc (aprox 3.2kb) into the 3' untranslated region of the fifth exon of the human POU5F1 gene