pGL3-Control-miR-20-Control
(Plasmid #21169)

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Depositing Lab

Joshua Mendell
Publication

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43. ( How to cite )
Sequence Information
- Sequences (2)

Full plasmid sequence is not available for this item.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	21169	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pGL3-Control
(Search Vector Database)
Backbone manufacturer
Promega
Backbone size w/o insert (bp) 5523
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
miR-20 Sensor
Species
H. sapiens (human)
Insert Size (bp)
66
Mutation
Sensor construct insert has two miR-20 binding site copies

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site XbaI (destroyed during cloning)
3′ cloning site XbaI (destroyed during cloning)
5′ sequencing primer GL2sequencing2
3′ sequencing primer RVprimer4

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA
- Luciferase Limited Use Label License

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pGL3-Control-miR-20-Control was a gift from Joshua Mendell (Addgene plasmid # 21169 ; http://n2t.net/addgene:21169 ; RRID:Addgene_21169)
For your References section:

c-Myc-regulated microRNAs modulate E2F1 expression. O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. Nature. 2005 Jun 9. 435(7043):839-43. 10.1038/nature03677 PubMed 15944709

pGL3-Control-miR-20-Control (Plasmid #21169)