pGL3-Control-miR-20-Control
(Plasmid #21169)

• Depositing Lab: Joshua Mendell
• Publication: O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	21169	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGL3-Control
  (Search Vector Database)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5523
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-20 Sensor
• Species: H. sapiens (human)
• Insert Size (bp): 66
• Mutation: Sensor construct insert has two miR-20 binding site copies

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: GL2sequencing2
• 3′ sequencing primer: RVprimer4

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License