|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21228||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7338
Selectable markersBlasticidin resistanct marker driven by Rex-1 promoter
Growth in Bacteria
Gene/Insert namealpha myosin heavy chain promoter
Alt nameaMHC promoter
SpeciesM. musculus (mouse)
Insert Size (bp)5470
/ Fusion Protein
- mCherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CTT GAA GCG CAT GAA CTC - 3' Sequencing only (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:aMHC-mCherry-Rex-Blasticidin was a gift from Mark Mercola (Addgene plasmid # 21228 ; http://n2t.net/addgene:21228 ; RRID:Addgene_21228)
For your References section:Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. PLoS ONE. 2009 . 4(4):e5046. 10.1371/journal.pone.0005046 PubMed 19352491