Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21294||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS+
- Backbone size w/o insert (bp) 3000
Vector typePromoter can be used to make transgenic mice
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHoxb7 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)1400
Entrez GeneHoxb7 (a.k.a. RP23-85A17.7, AI325018, Hox-2.3)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byJacqueline Deschamps
Terms and Licenses
- Not Available to Industry
Hoxb7 promoter #25, DNA #M126. The Hoxb7promoter fragment (sequences from −1316 to +81 of the Hoxb7gene), obtained from Dr Jacqueline Deschamps, was excised from the pGEM-Blue vector with KpnI and AvaI,
the ends blunted, and recloned into the EcoRV site of pBluescript KS+ in both orientations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Hoxb7 promoter was a gift from Frank Costantini (Addgene plasmid # 21294 ; http://n2t.net/addgene:21294 ; RRID:Addgene_21294)
For your References section:Dominant effects of RET receptor misexpression and ligand-independent RET signaling on ureteric bud development. Srinivas S, Wu Z, Chen CM, D'Agati V, Costantini F. Development. 1999 Apr . 126(7):1375-86. PubMed 10068631