Hoxb7 promoter
(Plasmid #21294)

• Depositing Lab: Frank Costantini
• Publication: Srinivas et al Development. 1999 Apr . 126(7):1375-86.

Backbone

• Vector backbone: pBluescript KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Promoter can be used to make transgenic mice

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hoxb7 promoter
• Alt name: Hoxb7
• Species: M. musculus (mouse)
• Insert Size (bp): 1400
• Entrez Gene: Hoxb7 (a.k.a. RP23-85A17.7, AI325018, Hox-2.3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jacqueline Deschamps

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hoxb7 promoter #25, DNA #M126. The Hoxb7promoter fragment (sequences from −1316 to +81 of the Hoxb7gene), obtained from Dr Jacqueline Deschamps, was excised from the pGEM-Blue vector with KpnI and AvaI,
the ends blunted, and recloned into the EcoRV site of pBluescript KS+ in both orientations.