AAVS1-eTLR
(Plasmid #214737)

• Purpose: encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
• Depositing Lab: Gil Westmeyer
• Publication: Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w.

Backbone

• Vector backbone: pBlueScript
• Backbone size w/o insert (bp): 3948
• Total vector size (bp): 11147
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AAVS1-eTLR
• Species: H. sapiens (human), G. gallus (chicken), B. taurus (bovine), O. cuniculus (rabbit), Synthetic; CMV, Anguilla japonica, influenza
• Insert Size (bp): 7199
• Entrez Gene: PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)

Resource Information

• Supplemental Documents:
  • AAVS1-eTLR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry