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pJA256
(Plasmid #21509)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21509 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONR P2R-P3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2639
  • Vector type
    MultiSite Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1223

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP2R (destroyed during cloning)
  • 3′ cloning site attP3 (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R/ T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The tbb-2 3'UTR sequence was taken from pCM1.36.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJA256 was a gift from Julie Ahringer (Addgene plasmid # 21509 ; http://n2t.net/addgene:21509 ; RRID:Addgene_21509)
  • For your References section:

    MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J. PLoS One. 2011;6(5):e20082. Epub 2011 May 26. 10.1371/journal.pone.0020082 PubMed 21637852