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pJA281
(Plasmid #22513)

Backbone

  • Vector backbone
    pDONRP4P1R
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2646
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    Pmex-5::mCherry (w introns w/o stop)
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1365

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP4 (destroyed during cloning)
  • 3′ cloning site attP1R (destroyed during cloning)
  • 5′ sequencing primer M13F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJA281 was a gift from Julie Ahringer (Addgene plasmid # 22513)
  • For your References section:

    MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J. PLoS One. 2011;6(5):e20082. Epub 2011 May 26. 10.1371/journal.pone.0020082 PubMed 21637852