Modified Shipping Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 23 - 27. If you have any questions, please contact us at

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at Learn more

(Plasmid #22513)

Add to Cart
Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2646
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Sequence Information


  • Gene/Insert name
    Pmex-5::mCherry (w introns w/o stop)
  • Species
    C. elegans (nematode)
  • Insert Size (bp)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP4 (destroyed during cloning)
  • 3′ cloning site attP1R (destroyed during cloning)
  • 5′ sequencing primer M13F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJA281 was a gift from Julie Ahringer (Addgene plasmid # 22513)
  • For your References section:

    MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J. PLoS One. 2011;6(5):e20082. Epub 2011 May 26. 10.1371/journal.pone.0020082 PubMed 21637852