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(Plasmid #21520)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 21520 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    contain aa254-503
  • GenBank ID
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

In our JCS paper (Li et al. J Cell Sci. 2008 Dec 15;121(Pt 24):4134-44), a new isoform of GW182 was identified with an extra N-terminal 253-amino-acid. That is why the name of these two clones changed from the original GW182D1a (aa1-250) and GW182D1(aa1-498) to GW1D1a(aa254-503) and GW1D1(aa254-751), respectively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-GW1D1a(aa254-503) was a gift from Edward Chan (Addgene plasmid # 21520 ; ; RRID:Addgene_21520)
  • For your References section:

    The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK. RNA. 2009 May . 15(5):804-13. 10.1261/rna.1229409 PubMed 19324964