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pLLNeo
(Plasmid #21617)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLLU2G
  • Backbone size (bp) 7861
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer BGH-rev
  • 3′ sequencing primer mPGK-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This is a 3rd generation lentiviral plasmid.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLLNeo was a gift from David Garbers (Addgene plasmid # 21617)
  • For your References section:

    Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation. Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH. Stem Cells. 2008 Nov . 26(11):2928-37. 10.1634/stemcells.2008-0134 PubMed 18719224