Addgene - pLLNeo Plasmid Data

Plasmid 21617: pLLNeo

Gene/insert name:
None
Vector backbone:
pLLU2G
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size (bp):
7861
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
BGH-rev List of Sequencing Primers
3' sequencing primer:
mPGK-F
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
David Garbers
Terms and Licenses:
MTA

Comments:

This is a 3rd generation lentiviral plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation. Dann et al (Stem Cells. 2008 Nov . 26(11):2928-37. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21617" in your Materials and Methods section.