|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21617||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7861
Vector typeLentiviral, RNAi
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer BGH-rev
- 3′ sequencing primer mPGK-F (Common Sequencing Primers)
This is a 3rd generation lentiviral plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLLNeo was a gift from David Garbers (Addgene plasmid # 21617 ; http://n2t.net/addgene:21617 ; RRID:Addgene_21617)
For your References section:Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation. Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH. Stem Cells. 2008 Nov . 26(11):2928-37. 10.1634/stemcells.2008-0134 PubMed 18719224