Addgene - Clathrin-LCa-EYFP Plasmid Data

Plasmid 21741: Clathrin-LCa-EYFP

Gene/insert name:
Clathrin, light polypeptide (LCa)
Alt name:
Clta
Insert size:
657
Species:
M. musculus (mouse)
GenBank ID:
NM_001080385
Entrez Gene:
Clta (RP23-209M8.4, AV026556)
Fusion protein or tag:
EYFP
Terminal:
C terminal on backbone
Vector backbone:
pEYFP-N1
(Search Vector Database)
Backbone manufacturer:
Clonetech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Cloning site 5':
Kpn1
Site destroyed during cloning:
No
Cloning site 3':
Age1
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-N, Clonetech# 6479-1 List of Sequencing Primers
3' sequencing primer:
EGFP-C, Clonetech# 6478-1
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Gift of J.H. Keen, Nat. Cell Biol. 1,1,(1999)
Sequence: View sequences (2)
Principal Investigator:
Xiaowei Zhuang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Chen et al (Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):11790-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21741" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14435: mRFP-Clc

12674: GFP-rab11 WT

12605: GFP-rab7 WT

21742: Clathrin-LCa-ECFP

14437: mRFP-Rab5