Growth in Bacteria
Growth instructionsWe recommend that it be streaked on an LB plate containing 12.5 µg/ml tetracycline. (Note that tetracycline stock can be made up at 12.5 mg/ml in 80% ethanol and stored at -20° C.
Full plasmid sequence is available only if provided by the depositing laboratory.
F- ∆hisB463 ∆(gpt-proAB-arg-lac)XIII zaj::Tn10
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Strain KJ1C was a gift from Keith Joung (Addgene plasmid # 21874)
Map generated by Addgene from plasmid data.