Growth in Bacteria
Growth instructionsCSH100 is not resistant to any antibiotics. We recommend that it be streaked out on an M9 minimal plate (with glucose) in order to maintain selection for the F’.
Full plasmid sequence is available only if provided by the depositing laboratory.
F’ lac proA+proB+(lacIq lacPL8)/ara- ∆(gpt-lac)5
This strain will be shipped as bacteria in an LB stab. Upon receipt, requesting scientists should restreak the strain on an M9 minimal plate. After restreaking on M9 to confirm the presence of the F', scientists can grow the strain in liquid LB.
M9 minimal medium agar plates: To prepare 500 ml, autoclave 439 ml H2O
with 7.5 g Bacto-agar and a stir bar. When agar has cooled to approximately
65°C, add 50 ml 10X M9 salts, 1 ml 1 M MgSO4, 10 ml 20% (w/v) glucose and 0.5 ml
100mM CaCl2 and then pour plates. Plates can be stored indefinitely at 4°C in
sealed plastic bags. (Alternatively, M9 plates can be purchased from Teknonva: http://www.teknova.com/product-p/m1260.htm
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Strain CSH100 was a gift from Keith Joung (Addgene plasmid # 21875)
Map generated by Addgene from plasmid data.