Addgene - Strain CSH100 Plasmid Data

Bacterial strain 21875: Strain CSH100

Gene/insert name:
CSH100 bacterial Strain
Vector backbone:
n/a
(Search Vector Database)
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
None
Growth strain(s)
CSH100
Growth temperature (℃):
37
Growth instructions:
CSH100 is not resistant to any antibiotics. We recommend that it be streaked out on an M9 minimal plate (with glucose) in order to maintain selection for the F’.
High or low copy:
Unknown
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

F’ lac proA+proB+(lacIq lacPL8)/ara- ∆(gpt-lac)5

This strain will be shipped as bacteria in an LB stab. Upon receipt, requesting scientists should restreak the strain on an M9 minimal plate. After restreaking on M9 to confirm the presence of the F', scientists can grow the strain in liquid LB.

M9 minimal medium agar plates: To prepare 500 ml, autoclave 439 ml H2O
with 7.5 g Bacto-agar and a stir bar. When agar has cooled to approximately
65°C, add 50 ml 10X M9 salts, 1 ml 1 M MgSO4, 10 ml 20% (w/v) glucose and 0.5 ml
100mM CaCl2 and then pour plates. Plates can be stored indefinitely at 4°C in
sealed plastic bags. (Alternatively, M9 plates can be purchased from Teknonva: http://www.teknova.com/product-p/m1260.htm

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21875" in your Materials and Methods section.