|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22089||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK (-)
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
Insert Size (bp)800
Entrez GeneFGF8 (a.k.a. FGF-8)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region.
For antisense probe linearize with EcoRI and use T7 Polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:cFGF8 was a gift from Gail Martin (Addgene plasmid # 22089 ; http://n2t.net/addgene:22089 ; RRID:Addgene_22089)
For your References section:Roles for FGF8 in the induction, initiation, and maintenance of chick limb development. Crossley PH, Minowada G, MacArthur CA, Martin GR. Cell. 1996 Jan 12. 84(1):127-36. 10.1016/S0092-8674(00)80999-X PubMed 8548816
Map uploaded by the depositor.