pcU6.3_gRNA_c1
(Plasmid
#221381)
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Purpose(Empty Backbone) Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
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Depositing Labs
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Depositing OrganizationFrancis Crick Institute
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221381 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcU6.3
- Backbone size (bp) 3518
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Modifications to backbone10x capture sequence 1 insertion
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Vector typeCRISPR
- Promoter G. gallus U6.3
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BsmBI cut sites flanking the gRNA spacer. gRNA to be inserted.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcU6.3_gRNA_c1 was a gift from James Briscoe & Tatjana Sauka-Spengler (Addgene plasmid # 221381 ; http://n2t.net/addgene:221381 ; RRID:Addgene_221381)
