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pTER_MDC1 (422-2)
(Plasmid #22191)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22191 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4358
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Bleocin (Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sh Mediator of DNA damage checkpoint 1
  • Alt name
    shMDC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    58

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer H1
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTER_MDC1 (422-2) was a gift from Eric Campeau (Addgene plasmid # 22191 ; http://n2t.net/addgene:22191 ; RRID:Addgene_22191)