Addgene - MDC1 in pENTR4 (330-5) Plasmid Data

Plasmid 26427: MDC1 in pENTR4 (330-5)

Gene/insert name:
Mediator of DNA damage checkpoint 1
Alt name:
MDC1
Alt name:
NFBD1
Alt name:
KIAA0170
Insert size:
6269
Species:
H. sapiens (human)
Entrez Gene:
MDC1 (DAAP-285E11.7, NFBD1)
Vector backbone:
pENTR4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Entry vector
Backbone size w/o insert (bp):
2477
Cloning site 5':
Sal I
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
MDC1 cDNA was derived from clone KIAA0170 (Kazusa)
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26427" in your Materials and Methods section.