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pENTR-GFP-MDC1 (770-7)
(Plasmid #26284)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26284 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3270
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mediator of DNA damage checkpoint 1
  • Alt name
    MDC1
  • Alt name
    NFBD1
  • Alt name
    KIAA0170
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6269
  • Entrez Gene
    MDC1 (a.k.a. NFBD1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bst BI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The functionality of the GFP-MDC1 protein was verified by co-localization with gamma-H2AX after DNA damage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-GFP-MDC1 (770-7) was a gift from Eric Campeau (Addgene plasmid # 26284)