Addgene - pLenti CMV/TO GFP-MDC1 (779-2) Plasmid Data

Plasmid 26285: pLenti CMV/TO GFP-MDC1 (779-2)

Gene/insert name:
Mediator of DNA damage checkpoint 1
Alt name:
MDC1
Alt name:
NFBD1
Alt name:
KIAA0170
Insert size:
6269
Species:
H. sapiens (human)
Entrez Gene:
MDC1 (DAAP-285E11.7, NFBD1)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7796
Cloning site 5':
Bst BI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
WPRErev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers, other:
GFP
Person or lab that originally
cloned the gene/insert:
MDC1 cDNA was derived from the clone KIAA0170 (Kazusa).
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Functionality of the GFP-MDC1 fusion protein was verified by co-localization with gamma-H2AX after DNA damage.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26285" in your Materials and Methods section.