Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pLenti CMV/TO GFP-MDC1 (779-2)
(Plasmid #26285)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26285 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7796
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mediator of DNA damage checkpoint 1
  • Alt name
    MDC1
  • Alt name
    NFBD1
  • Alt name
    KIAA0170
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6269
  • Entrez Gene
    MDC1 (a.k.a. NFBD1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bst BI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer CMVforw
  • 3′ sequencing primer WPRErev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MDC1 cDNA was derived from the clone KIAA0170 (Kazusa).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Functionality of the GFP-MDC1 fusion protein was verified by co-localization with gamma-H2AX after DNA damage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV/TO GFP-MDC1 (779-2) was a gift from Eric Campeau (Addgene plasmid # 26285 ; http://n2t.net/addgene:26285 ; RRID:Addgene_26285)