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SCP2-CMVenh-luc
(Plasmid #22230)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22230 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    a MODIFIED version (constructed by us) of Invitrogen’s pRC/CMV
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCP2 (super core promoter 2)-CMVenh luciferase
  • Species
    H. sapiens (human), D. melanogaster (fly); viral and luciferase is from firefly
  • Insert Size (bp)
    81

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer for sequencing the luc gene use T7; for sequencing the enhancer/
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SCP2-CMVenh-luc was a gift from James Kadonaga (Addgene plasmid # 22230 ; http://n2t.net/addgene:22230 ; RRID:Addgene_22230)
  • For your References section:

    Rational design of a super core promoter that enhances gene expression. Juven-Gershon T, Cheng S, Kadonaga JT. Nat Methods. 2006 Nov . 3(11):917-22. 10.1038/nmeth937 PubMed 17124735