Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

E7 in pENTR1A (w186-1)
(Plasmid #22254)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Human papilloma virus E7
  • Alt name
    E7
  • Insert Size (bp)
    296
  • Entrez Gene
    E7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Xho I (destroyed during cloning)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E7 cDNA was obtained from Dr. Christian Beausejour.
  • Terms and Licenses

Depositor Comments

The E7 protein binds and degrades the Retinoblastoma protein. It also binds to Mi2beta-HDAC1 from the NURD complex (Brehm et al. 1999).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E7 in pENTR1A (w186-1) was a gift from Eric Campeau (Addgene plasmid # 22254)