Addgene - E7 in pENTR1A (w186-1) Plasmid Data

Plasmid 22254: E7 in pENTR1A (w186-1)

Gene/insert name:
Human papilloma virus E7
Alt name:
E7
Insert size:
296
Entrez Gene:
E7 ()
Vector backbone:
pENTR1A
(Search Vector Database)
Vector type:
Entry vector
Backbone size w/o insert (bp):
2298
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xho I
Site destroyed during cloning:
Yes
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
E7 cDNA was obtained from Dr. Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

The E7 protein binds and degrades the Retinoblastoma protein. It also binds to Mi2beta-HDAC1 from the NURD complex (Brehm et al. 1999).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22254" in your Materials and Methods section.