|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22456||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeExpression vector
Growth in Bacteria
SpeciesD. rerio (zebrafish)
Insert Size (bp)1300
Entrez Genenotch3 (a.k.a. fa14b08, notch5, wu:fa14b08)
/ Fusion Protein
- mCherry (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, SP6
- 3′ sequencing primer EBV-Rev (Common Sequencing Primers)
Generated by LR reaction between pCSCherryDest and pENTRnotch3icd
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSCherryN5icd was a gift from Nathan Lawson (Addgene plasmid # 22456)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311
Generated by Addgene from full sequence supplied by depositor.