Addgene - pCSCherryN5icd Plasmid Data

Plasmid 22456: pCSCherryN5icd

Gene/insert name:
Notch3
Insert size:
1300
Species:
D. rerio (zebrafish)
Entrez Gene:
notch3 (notch5, fa14b08, wu:fa14b08)
Fusion protein or tag:
mCherry
Terminal:
N terminal on insert
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Expression vector
Backbone size w/o insert (bp):
5000
5' sequencing primer:
CMV-F, SP6 List of Sequencing Primers
3' sequencing primer:
EBV-Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

Generated by LR reaction between pCSCherryDest and pENTRnotch3icd

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc et al (Dev Dyn. 2007 Nov . 236(11):3077-87. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22456" in your Materials and Methods section.