Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22467||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeInjection construct
Growth in Bacteria
SpeciesD. rerio (zebrafish)
Insert Size (bp)1300
Entrez Genenotch3 (a.k.a. fa14b08, notch5, wu:fa14b08)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Gateway LR reaction between pCSEGFPDest and pENTRnotch5icd
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSegfpN5ICD was a gift from Nathan Lawson (Addgene plasmid # 22467 ; http://n2t.net/addgene:22467 ; RRID:Addgene_22467)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311