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Purposeexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62542 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2-nCas9n
- Backbone size w/o insert (bp) 8272
- Total vector size (bp) 8910
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenanos 3' UTR
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Alt namenos1
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SpeciesD. rerio (zebrafish)
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Mutation3' UTR only
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Entrez Genenanos3 (a.k.a. cb725, nanos, nanos1, nos1)
- Promoter SP6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer ATCaccggtAGCGGACATTGATGCTCC
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
vector backbone was Addgene Plasmid #47929 from Wenbiao Chen
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-nCas9n-nanos 3’UTR was a gift from Antonio Giraldez (Addgene plasmid # 62542 ; http://n2t.net/addgene:62542 ; RRID:Addgene_62542) -
For your References section:
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, Khokha MK, Giraldez AJ. Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543. 10.1038/nmeth.3543 PubMed 26322839