Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pRSVBneoT
(Plasmid #22883)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22883 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM3zf(+)
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Early region SV40
  • Species
    SV40
  • Insert Size (bp)
    2658

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was made more than 20 years ago. The vector (pGEM3zf(+)) originated from Promega while the cassette (pMCneo(PolyA)) from Stratagene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSVBneoT was a gift from James Pipas (Addgene plasmid # 22883 ; http://n2t.net/addgene:22883 ; RRID:Addgene_22883)
  • For your References section:

    The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain. Srinivasan A, McClellan AJ, Vartikar J, Marks I, Cantalupo P, Li Y, Whyte P, Rundell K, Brodsky JL, Pipas JM. Mol Cell Biol. 1997 Aug . 17(8):4761-73. PubMed 9234732