Addgene - pKAR1 Plasmid Data

Plasmid 23104: pKAR1

Gene/insert name:
None
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pUHD-P3
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi
Backbone size (bp):
4604
Modifications to Backbone:
N/A
5' sequencing primer:
LNCX List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
EGFP
Sequence: View sequences (3)
Principal Investigator:
Randy Poon
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An inducible system for expression and validation of the specificity of short hairpin RNA in mammalian cells. Ma et al (Nucleic Acids Res. 2007 . 35(4):e22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23104" in your Materials and Methods section.