|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23336||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameEnhanced Circularly Permuted Outer Membrane Protein X
Insert Size (bp)600
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer See sequence (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB33eCPX was a gift from Patrick Daugherty (Addgene plasmid # 23336 ; http://n2t.net/addgene:23336 ; RRID:Addgene_23336)
For your References section:Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides. Rice JJ, Daugherty PS. Protein Eng Des Sel. 2008 Jul . 21(7):435-42. 10.1093/protein/gzn020 PubMed 18480093