YFP-Parkin
(Plasmid #23955)

• Purpose: Mammalian expression of human Park2 fused to EYFP
• Depositing Lab: Richard Youle
• Publication: Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Backbone

• Vector backbone: eYFP-C1 (clontech)
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Park2
• Alt name: Parkin
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NM_004562
• Entrez Gene: PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
• Tag / Fusion Protein:
  • eYFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 38 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

first ATG is deleted