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mCherry-Parkin
(Plasmid #23956)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    mCherry-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Park2
  • Alt name
    Parkin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • GenBank ID
    NM_004562
  • Entrez Gene
    PARK2 (a.k.a. KB-152G3.1, AR-JP, LPRS2, PDJ, PRKN)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

first ATG is deleted

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Parkin was a gift from Richard Youle (Addgene plasmid # 23956)
  • For your References section:

    Parkin is recruited selectively to impaired mitochondria and promotes their autophagy. Narendra D, Tanaka A, Suen DF, Youle RJ. J Cell Biol. 2008 Dec 1. 183(5):795-803. 10.1083/jcb.200809125 PubMed 19029340