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peZinCh-1
(Plasmid #24172)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24172 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    nova blue (for DNA amplification)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eZinCh-1
  • Insert Size (bp)
    1800
  • Mutation
    Cerulean and Citrine both contain mutations Y39H and S208C
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peZinCh-1 was a gift from Maarten Merkx (Addgene plasmid # 24172 ; http://n2t.net/addgene:24172 ; RRID:Addgene_24172)
  • For your References section:

    Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M. Nat Methods. 2009 Aug 30. ():. 10.1038/nmeth.1368 PubMed 19718032