|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24178||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9956
Vector typeMammalian Expression, Lentiviral ; Gateway
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB survival strain
Copy numberLow Copy
Gene/Insert nameChloramphenicol resistance gene (CmR) - ccdB gene
SpeciesChloramphenicol resistance gene (CmR) - ccdB gene
/ Fusion Protein
- DsRED (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer sequence
- 3′ sequencing primer sequence (Common Sequencing Primers)
V5 tag deleted
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLVU/RED was a gift from Lars Ittner (Addgene plasmid # 24178 ; http://n2t.net/addgene:24178 ; RRID:Addgene_24178)
For your References section:Gateway-compatible lentiviral transfer vectors for ubiquitin promoter driven expression of fluorescent fusion proteins. Krupka N, Strappe P, Götz J, Ittner LM. Plasmid. 2010 Feb 2. ():. 10.1016/j.plasmid.2010.01.002 PubMed 20132838
Map uploaded by the depositor.