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(Plasmid #24348)


Item Catalog # Description Quantity Price (USD)
Plasmid 24348 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Synthetic renilla luciferase
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc65I (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AC5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Synthetic Renilla luciferase that was codon optimized for expression in mammalian
cells (hRluc) was amplified using primers PR444
(ataaGCGGCCGCTTACTGCTCGTTCTTCAGCA), and pGL4.75 (hRluc/CMV, Promega, catalog #
E6931) as the template. The PCR product was cloned into pPAC5C-PL using Acc65I and NotI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-hRluc was a gift from Liqun Luo (Addgene plasmid # 24348 ; ; RRID:Addgene_24348)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990