- Backbone size w/o insert (bp) 6500
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameSynthetic renilla luciferase
Insert Size (bp)900
- Cloning method Restriction Enzyme
- 5′ cloning site Acc65I (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer AC5 (Common Sequencing Primers)
Synthetic Renilla luciferase that was codon optimized for expression in mammalian
cells (hRluc) was amplified using primers PR444
(ataaGGTACCaaaATGGCTTCCAAGGTGTACGA) and PR443
(ataaGCGGCCGCTTACTGCTCGTTCTTCAGCA), and pGL4.75 (hRluc/CMV, Promega, catalog #
E6931) as the template. The PCR product was cloned into pPAC5C-PL using Acc65I and NotI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC-hRluc was a gift from Liqun Luo (Addgene plasmid # 24348)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.