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Plasmid 24348: pAC-hRluc
  • hRluc

  • Synthetic renilla luciferase

  • 900

  • Synthetic

  • pPac5c-PL
    (Search Vector Database)

  • Insect Expression

  • 6500

  • Acc65I

  • No

  • NotI

  • No

  • AC5 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • View map

  • pAC-hRluc (GenBank)

  • Liqun Luo

  • MTA
    Luciferase Limited Use Label License

Comments: 

Synthetic Renilla luciferase that was codon optimized for expression in mammalian
cells (hRluc) was amplified using primers PR444
(ataaGGTACCaaaATGGCTTCCAAGGTGTACGA) and PR443
(ataaGCGGCCGCTTACTGCTCGTTCTTCAGCA), and pGL4.75 (hRluc/CMV, Promega, catalog #
E6931) as the template. The PCR product was cloned into pPAC5C-PL using Acc65I and NotI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24348" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only