Addgene - pAC-hRluc Plasmid Data

Plasmid 24348: pAC-hRluc

Gene/insert name:
hRluc
Alt name:
Synthetic renilla luciferase
Insert size:
900
Species:
Synthetic
Vector backbone:
pPac5c-PL
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
6500
Cloning site 5':
Acc65I
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
AC5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pAC-hRluc (GenBank)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Synthetic Renilla luciferase that was codon optimized for expression in mammalian
cells (hRluc) was amplified using primers PR444
(ataaGGTACCaaaATGGCTTCCAAGGTGTACGA) and PR443
(ataaGCGGCCGCTTACTGCTCGTTCTTCAGCA), and pGL4.75 (hRluc/CMV, Promega, catalog #
E6931) as the template. The PCR product was cloned into pPAC5C-PL using Acc65I and NotI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24348" in your Materials and Methods section.

Price: $65.00

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