Addgene - pUAST>stop>mCD8-GFP Plasmid Data

Plasmid 24385: pUAST>stop>mCD8-GFP

Gene/insert name:
mCD8-GFP
Insert size:
3000
Vector backbone:
pUAS-mCD8-GFP
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
9000
5' sequencing primer:
GFP-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (4)
Map:
View map
Supplemental document:
pUAST>stop>mCD8-GFP (GenBank)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA

Comments:

The FRT-stop-FRT cassette, with additional BglII/NotI restriction sites, was PCR amplified from genomic DNA of UAS-FRT-stop-FRT-shibire (ts) flies and cloned into pUAST-mCD8-gfp to generate pUAST-FRT-stop-FRT-mCD8-gfp (pUAS>stop>mCD8-GFP)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24385" in your Materials and Methods section.