Addgene - pcDNA3-Ro2 Plasmid Data

Plasmid 24503: pcDNA3-Ro2

Gene/insert name:
RASSL Ro2
Alt name:
Ro2
Alt name:
KOR
Species:
H. sapiens (human)
Entrez Gene:
OPRK1 (KOR, OPRK)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Fusion protein or tag:
NLS from prolactin
Terminal:
N terminal on insert
Mutation:
mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
KpnI/XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Bruce Conklin
Terms and Licenses:
MTA

Comments:

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt

Prolactin NLS sequence: MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS

For more information about this plasmid see: http://openwetware.org/wiki/R02-ORF

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlling signaling with a specifically designed Gi-coupled receptor. Coward et al (Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24503" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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