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(Plasmid #24503)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 24503 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    RASSL Ro2
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    mutations in extracellular loops 2 (see comments below) and 3 (E297Q), making it a RASSL.
  • Entrez Gene
    OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • NLS from prolactin (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI/XhoI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt


For more information about this plasmid see:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Ro2 was a gift from Bruce Conklin (Addgene plasmid # 24503 ; ; RRID:Addgene_24503)
  • For your References section:

    Controlling signaling with a specifically designed Gi-coupled receptor. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR.. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. 10.1073/pnas.95.1.352 PubMed 9419379