|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24504||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRASSL Ro1
Alt nameOPRK1, KOR, OPRK
SpeciesH. sapiens (human)
Mutationmutations in extracellular loop 2, making it a RASSL (see comments below).
Entrez GeneOPRK1 (a.k.a. K-OR-1, KOR, KOR-1, OPRK)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, T7 (Common Sequencing Primers)
Terms and Licenses
Extracellular Loop 2 (EL2) mutations shown in lowercase: aSSVGISAIVLGvTqpRdgavvCtLQFPspswyWDtvt
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Ro1 was a gift from Bruce Conklin (Addgene plasmid # 24504 ; http://n2t.net/addgene:24504 ; RRID:Addgene_24504)