Addgene - pEGFP-N1-TFEB Plasmid Data

Plasmid 38119: pEGFP-N1-TFEB

Gene/insert name:
TFEB
Alt name:
Transcription factor EB
Insert size:
1428
Species:
H. sapiens (human)
GenBank ID:
NM_007162.2
Entrez Gene:
TFEB (RP4-696P19.3, ALPHATFEB, BHLHE35, TCFEB)
Fusion protein or tag:
EGFP
Terminal:
C terminal on insert
Mutation:
deleted stop codon
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Glontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Promoter:
CMV
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
CGCAAATGGGCGGTAGGCGTG List of Sequencing Primers
3' sequencing primer:
CGTCGCCGTCCAGCTCGACCAG
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Andrea Ballabio, TIGEM, Naples, Italy
Sequence: View sequences (2)
Principal Investigator:
Shawn Ferguson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Transcription Factor TFEB Links mTORC1 Signaling to Transcriptional Control of Lysosome Homeostasis. Roczniak-Ferguson et al (Sci Signal. 2012 Jun 12;5(228):ra42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38119" in your Materials and Methods section.