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pEGFP-N1-delta30-TFEB
(Plasmid #44445)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44445 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Glontech
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 6061
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TFEB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1321
  • Mutation
    Deleted amino acids 1-30; H32D
  • Entrez Gene
    TFEB (a.k.a. ALPHATFEB, BHLHE35, TCFEB)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer CGTCGCCGTCCAGCTCGACCAG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrea Ballabio, TIGEM, Naples, Italy
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The first 30 amino acids of the TFEB insert (up to M31) were deleted. In order to create an optimal Kozak sequence at the new start site (M31), an additional mutation was introduced that changed the second amino acid from H to D (H32D mutation relative to the wild-type sequence).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-delta30-TFEB was a gift from Shawn Ferguson (Addgene plasmid # 44445 ; http://n2t.net/addgene:44445 ; RRID:Addgene_44445)
  • For your References section:

    The Transcription Factor TFEB Links mTORC1 Signaling to Transcriptional Control of Lysosome Homeostasis. Roczniak-Ferguson A, Petit CS, Froehlich F, Qian S, Ky J, Angarola B, Walther TC, Ferguson SM. Sci Signal. 2012 Jun 12;5(228):ra42. 10.1126/scisignal.2002790 PubMed 22692423