Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24533||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePAK binding domain
SpeciesH. sapiens (human)
Insert Size (bp)300
Entrez GenePAK1 (a.k.a. IDDMSSD, PAKalpha)
/ Fusion Protein
- Ypet (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-Ypet-PBD was a gift from Klaus Hahn (Addgene plasmid # 24533 ; http://n2t.net/addgene:24533 ; RRID:Addgene_24533)
For your References section:Coordination of Rho GTPase activities during cell protrusion. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell A, Johnson GL, Hahn KM, Danuser G. Nature. 2009 Sep 3. 461(7260):99-103. 10.1038/nature08242 PubMed 19693013