MW005 (bacterial strain)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||24545||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth instructionsStrain must be grown at 32oC or less (30oC is fine)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7649 galU galK rspL nupG [ λcI857 (cro-bioA) < > araC-PBADtrfA]
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MW005 (bacterial strain) was a gift from Colin Dolphin (Addgene plasmid # 24545)
For your References section:Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host. Westenberg M, Bamps S, Soedling H, Hope IA, Dolphin CT. BMC Biotechnol. 2010 Mar 29. 10(1):27. 10.1186/1472-6750-10-27 PubMed 20350301