Addgene - pSICO-Flpo Plasmid Data

Plasmid 24969: pSICO-Flpo

Gene/insert name:
Flp recombinase
Insert size:
1300
Vector backbone:
pSico
(Search Vector Database)
Backbone manufacturer:
Addgene Plasmid 11578
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
6739
Cloning site 5':
StuI
Site destroyed during cloning:
No
Cloning site 3':
HpaI
Site destroyed during cloning:
No
5' sequencing primer:
mPGK-F List of Sequencing Primers
3' sequencing primer:
WPRE-R (CATAGCGTAAAAGGAGCAACA)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

pSico-Flpo allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Young et al (Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24969" in your Materials and Methods section.