pCI-neo-sOVA
(Plasmid #25098)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    soluble ovalbumin
  • Alt name
    OVA
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer T7 EEV Promoter Primer (Promega Cat.# Q6700)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-sOVA was a gift from Maria Castro (Addgene plasmid # 25098)
  • For your References section:

    Kupfer-type immunological synapse characteristics do not predict anti-brain tumor cytolytic T-cell function in vivo. Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein PR. Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21. 10.1073/pnas.0911587107 PubMed 20133734