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pCI-neo-cOVA
(Plasmid #25097)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25097 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytoplasmic ovalbumin
  • Alt name
    OVA
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    789

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer T7 EEV Promoter Primer (Promega Cat.# Q6700)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-cOVA was a gift from Maria Castro (Addgene plasmid # 25097 ; http://n2t.net/addgene:25097 ; RRID:Addgene_25097)
  • For your References section:

    Kupfer-type immunological synapse characteristics do not predict anti-brain tumor cytolytic T-cell function in vivo. Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein PR. Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21. 10.1073/pnas.0911587107 PubMed 20133734