|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31598||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneTCAE (modified)
Backbone manufacturerReff et al., 1994
- Backbone size w/o insert (bp) 9209
Vector typeMammalian Expression
Growth in Bacteria
SpeciesG. gallus (chicken)
Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
*The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOVA was a gift from Shoshana Levy (Addgene plasmid # 31598 ; http://n2t.net/addgene:31598 ; RRID:Addgene_31598)
For your References section:DNA vaccination with cytokine fusion constructs biases the immune response to ovalbumin. Maecker HT, Umetsu DT, DeKruyff RH, Levy S. Vaccine. 1997 Oct . 15(15):1687-96. 10.1016/S0264-410X(97)00088-1 PubMed 9364701
Map uploaded by the depositor.