Addgene - pWPI_SPbFGF Plasmid Data

Plasmid 25812: pWPI_SPbFGF

Gene/insert name:
pWPI_SPbFGF
Alt name:
FGF2
Insert size:
620
Species:
H. sapiens (human)
Entrez Gene:
FGF2 (BFGF, FGF-2, FGFB, HBGF-2)
Mutation:
Ig signal peptide in N-terminal of human FGF2
Vector backbone:
pWPI
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
11101
Cloning site 5':
PmeI
Site destroyed during cloning:
Yes
Cloning site 3':
PmeI
Site destroyed during cloning:
Yes
5' sequencing primer:
attctcaagcctcagacagt List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
HB101 or Top10 are recommended for growing lentivectors
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jozsef Kiss
Principal Investigator:
Patrick Salmon
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Expression of FGF-2 in neural progenitor cells enhances their potential for cellular brain repair in the rodent cortex. Dayer et al (Brain. 2007 Nov . 130(Pt 11):2962-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25812" in your Materials and Methods section.