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Plasmid 12270: pBABE puro SDF-1 alpha
  • SDF-1 alpha

  • stromal cell-derived factor 1

  • 400

  • H. sapiens (human)

  • CXCL12 (IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)

  • pBABE-puro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5150

  • BamHI

  • No

  • XhoI/SalI

  • Yes

  • pBABE 5' List of Sequencing Primers

  • pBABE 3'

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • See Shirozu M et al., 1995, Genomics 28: 495-500.

  • View sequences (1)
  • Bob Weinberg

  • UBMTA

Comments: 

A 0.4kb BamHI/XhoI fragment of the human SDF-1 alpha cDNA was cloned into the BamHI/SalI site of the pBABE-puro vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo et al (Cell. 2005 May 6. 121(3):335-48. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12270" in your Materials and Methods section.