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pBABE puro SDF-1 alpha
(Plasmid #12270)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SDF-1 alpha
  • Alt name
    stromal cell-derived factor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Entrez Gene
    CXCL12 (a.k.a. IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    See Shirozu M et al., 1995, Genomics 28: 495-500.
  • Terms and Licenses

Depositor Comments

A 0.4kb BamHI/XhoI fragment of the human SDF-1 alpha cDNA was cloned into the BamHI/SalI site of the pBABE-puro vector.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro SDF-1 alpha was a gift from Bob Weinberg (Addgene plasmid # 12270)
  • For your References section:

    Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA. Cell. 2005 May 6. 121(3):335-48. 10.1016/j.cell.2005.02.034 PubMed 15882617