Addgene - pLKO.1 puro GFP siRNA Plasmid Data

Plasmid 12273: pLKO.1 puro GFP siRNA

Gene/insert name:
GFP siRNA
Alt name:
siGFP
Insert size:
59
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7139
Cloning site 5':
AgeI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (3)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

siRNA directed against GFP: 5'-GCAAGCTGACCCTGAAGTTCA-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo et al (Cell. 2005 May 6. 121(3):335-48. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12273" in your Materials and Methods section.