pLKO.1 puro GFP siRNA
(Plasmid #12273)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7139
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    GFP siRNA
  • Alt name
    siGFP
  • Insert Size (bp)
    59

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

siRNA directed against GFP: 5'-GCAAGCTGACCCTGAAGTTCA-3'

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 puro GFP siRNA was a gift from Bob Weinberg (Addgene plasmid # 12273)
  • For your References section:

    Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA. Cell. 2005 May 6. 121(3):335-48. 10.1016/j.cell.2005.02.034 PubMed 15882617