pLKO.1 puro GFP siRNA
- Backbone size w/o insert (bp) 7139
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGFP siRNA
Insert Size (bp)59
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Terms and Licenses
siRNA directed against GFP: 5'-GCAAGCTGACCCTGAAGTTCA-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 puro GFP siRNA was a gift from Bob Weinberg (Addgene plasmid # 12273)
For your References section:Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA. Cell. 2005 May 6. 121(3):335-48. 10.1016/j.cell.2005.02.034 PubMed 15882617
Map generated by Addgene from full sequence.