Addgene - pLKO.1 puro CXCR4 siRNA-1 Plasmid Data

Plasmid 12271: pLKO.1 puro CXCR4 siRNA-1

Gene/insert name:
CXCR4 siRNA
Alt name:
CXCR4
Insert size:
59
Species:
H. sapiens (human)
Entrez Gene:
CXCR4 (CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM)
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7139
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

CXCR4-siRNA-1 sequence: 5'-TGGAGGGGATCAGTATATACA-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo et al (Cell. 2005 May 6. 121(3):335-48. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12271" in your Materials and Methods section.