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pcDNA3-PalmPalm-ClopHensor
(Plasmid #25940)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25940 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5344
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PalmPalm-ClopHensor
  • Alt name
    E2GFP fused to DsRed-monomer
  • Species
    Aq. Victoria and Discosoma sp
  • Insert Size (bp)
    1542
  • Mutation
    N-term (20AA) of Gap-43
  • Tags / Fusion Proteins
    • Gap20 (N terminal on insert)
    • Strep-II (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-PalmPalm-ClopHensor was a gift from Daniele Arosio (Addgene plasmid # 25940)
  • For your References section:

    Simultaneous intracellular chloride and pH measurements using a GFP-based sensor. Arosio D, Ricci F, Marchetti L, Gualdani R, Albertazzi L, Beltram F. Nat Methods. 2010 Jul . 7(7):516-8. 10.1038/nmeth.1471 PubMed 20581829