|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36856||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5694
- Total vector size (bp) 7137
Modifications to backbonea hygromysin resistance gene was inserted to facilitate selection for stably transfected cells.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefluorescent protein voltage sensor
Alt namesuper ecliptic pHluorin
Insert Size (bp)1443
MutationCi-VSP contains R217Q mutation; super ecliptic pHluorin contains A227D mutation. The A227D mutation increases the fluorescence response magnitude.
GenBank IDAB183035 AY533296
- Promoter simian CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GACGTAAATGGGCGGTAGGCGTG
- 3′ sequencing primer TTAAAAAACCTCCCACACCTC (Common Sequencing Primers)
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position Q239 in the CiVSP sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ArcLight-Q239 was a gift from Vincent Pieribone (Addgene plasmid # 36856 ; http://n2t.net/addgene:36856 ; RRID:Addgene_36856)
For your References section:Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Neuron. 2012 Sep 6;75(5):779-85. 10.1016/j.neuron.2012.06.040 PubMed 22958819