|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26027||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2858
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefli1a intron 1
SpeciesD. rerio (zebrafish)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
"p5E" refers to 5' entry clones flanked by L4-R1
There are a few mismatches between depositor's sequence and Addgene's sequence. The changes should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:475 p5EfliEBSbasP was a gift from Nathan Lawson (Addgene plasmid # 26027)
For your References section:A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub 2013 Mar 5. 10.1242/dev.084152 PubMed 23462469