Depositor Sequences: None.
Addgene Sequences: Partial (1)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26033||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeEntry vector
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)600
Entrez Genemir126a (a.k.a. dre-mir-126, dre-mir-126a, mir126)
- Cloning method Restriction Enzyme
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).
There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:776 pME-miR-126 was a gift from Nathan Lawson (Addgene plasmid # 26033)
For your References section:MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. Nature. 2010 Apr 22. 464(7292):1196-200. 10.1038/nature08889 PubMed 20364122
Generated by Addgene from plasmid data.