776 pME-miR-126
(Plasmid #26033)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    miR-126
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    600
  • Entrez Gene
    mir126 (a.k.a. dre-mir-126, mir126a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attL2 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).

There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    776 pME-miR-126 was a gift from Nathan Lawson (Addgene plasmid # 26033)
  • For your References section:

    MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. Nature. 2010 Apr 22. 464(7292):1196-200. 10.1038/nature08889 PubMed 20364122