Addgene - 776 pME-miR-126 Plasmid Data

Plasmid 26033: 776 pME-miR-126

Gene/insert name:
miR-126
Insert size:
600
Species:
D. rerio (zebrafish)
Entrez Gene:
mir126 (dre-mir-126, mir126a)
Vector backbone:
pME
(Search Vector Database)
Vector type:
Entry vector
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attL2
Site destroyed during cloning:
No
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

The insert includes genomic sequence flanking the mature miR-126 sequence on chromosome 8 (miRBase accession number MI0001979, Zv8).

There is a C->T mutation in the miR126. The mutation is probably due to a SNP and should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli et al (Nature. 2010 Apr 22. 464(7292):1196-200. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26033" in your Materials and Methods section.